Test Preparation : No special preparation required

Sample : Paraffin Blocks/Tissue in buffered formalin

Methodlogy : IHC

Description :

WHAT IS IT?

Lymphoid cells integrate a range of signals to start a particular immune response. These signals are produced by the coordinated interaction of many cell surfaces molecules with their opposing receptors. Lymphoid cells go through a complex process of maturation and activation as a result of the precise recognition of the antigen through antigen specific receptors and of the monitoring of their particular environments through the so-called coreceptor molecules, which contributes to the plasticity and sensitivity of immune response. The main protein with sialic acid on lymphocyte surfaces is called CD43. However, it is still completely unclear how exactly this protein functions in various lymphoid cells under physiologically normal circumstances.

WHY?

At the antigen locations that the primary antibody has targeted, a colorful reaction product precipitates during the immunostaining process. Before interpreting data, a skilled pathologist with experience in immunohistochemical methods must assess both positive and negative tissue controls. Positive tissue control: To ensure that all reagents are working properly, it is important to first inspect the stained positive tissue control. reactivity is shown by the presence of properly colored reaction products inside the target cells. Negative tissue control: It should be inspected following a positive tissue control to ensure that the main antibody specifically labeled the target antigen. The lack of antibody cross reactivity to cells or cellular components is confirmed by the absence of particular staining in the negative tissue control. The patient specimen and tissue examination should come last. Any background staining of a negative reagent control should be considered when assessing the strength of any positive staining.

PRECAUTIONS

Tissues that have undergone routine processing, are neutral-buffered formalin-fixed, paraffin embedded, and can be used with this primary antibody. The tissue fixative that is advised is 10% neutral-buffered formalin. Prolonged fixation or unique procedures, such as the decalcification of bone marrow preparations, may have variable effects. Each sample needs to be prepared by being sliced to the proper thickness (about 3 micrometers), then put on a glass slide that is negatively charged. The tissue section-containing slides can be roasted for at least two hours.