Test Preparation : No special preparation required

Sample : Paraffin Blocks/Tissue in buffered formalin

Methodlogy : IHC

Description :

WHAT IS IT?

All leucocytes display CD45 (lymphocyte common antigen), a receptor-linked protein tyrosine phosphatase that is essential to the operation of these cells. An antigen present on lymphoid cells is recognised by LCA antibodies. It reacts with granulocytes, monocytes, macrophages, T-cell subtypes, B-cells, and T-cell subtypes. When lymphoma is suspected, the CD (cluster of differentiation) is utilised to help with the differential diagnosis of undifferentiated neoplasms. Most lymphocytes contain this antibody, which is virtually exclusively expressed by cells of the hematopoietic lineage. Anti-CD45 LCA is therefore aimed against CD45 and can be used to help distinguish between healthy and aberrant leukocytic cells as well as to help with the diagnosis of anaplastic tumors.

WHY?

Antibody combinations enable the identification of particular cell types. measurement of cell differentiation level identifying aberrant cells. With just one plot, CD45 enables identification of the majority of significant populations. In determining a cell's immunophenotype, CD45 is helpful. During the stages of myeloma, it tends to manifest itself more in the plasma cells. When a cell is CD45+, CD45 is present on its surface; when it is CD45-, it does not exist.

PRECAUTIONS

There is no need for extra preparations. However, previous pharmaceutical use as well as any underlying illnesses or allergies should be revealed. The type of diagnostic test that should be performed is decided by the doctor taking into account variables such as the type of tumour suspected, signs, symptoms, age, medical problems, and the results of earlier medical tests. Accordingly, specific instructions will be provided.