Test Preparation : No special preparation required

Sample : Paraffin Blocks/Tissue in buffered formalin

Methodlogy : IHC

Description :

WHAT IS IT?

The term "IHC CD68 test" designates a particular type of immunohistochemistry (IHC) staining procedure utilising a CD68 antibody. A laboratory technique called immunohistochemistry is used to identify particular proteins in tissue slices. A typical marker for cells of the monocyte/macrophage lineage, a subset of immune cells involved in phagocytosis and the control of immunological response, is the glycoprotein CD68. An antibody that selectively binds to the CD68 protein is used to stain tissue sections for the IHC CD68 test. Under a microscope, this staining aids in spotting and locating CD68-expressing cells, mainly macrophages and monocytes, in the tissue sample. The existence and dispersion of macrophages in diverse tissues are evaluated using this method, which is frequently used in research, diagnostics, and pathology. This information can be utilised to understand various diseases, inflammation, and immunological responses.

WHY?

A crucial tool for biological research, diagnosis, and clinical practise is the immunohistochemistry (IHC) CD68 test. This test allows for the exact identification and localization of cells from the monocyte/macrophage lineage inside tissue samples by using targeted antibodies that specifically target the CD68 protein. This talent has broad ramifications. By exposing different patterns of macrophage dispersion, it assists in the diagnosis of a variety of illnesses, from infections to malignancies. Our understanding of immunological responses, inflammation, tissue healing, and disease progression is also improved by the information gleaned through IHC CD68 testing.

PRECAUTIONS

The Immunohistochemistry (IHC) CD68 test involves several steps to ensure accurate results. First, gather materials like tissue samples, slides, reagents, and equipment. Section tissue samples into thin slices, prepare slides, and use antigen retrieval methods. Block nonspecific binding sites with a blocking solution. Incubate tissue sections with the CD68 primary antibody, perform washes, apply secondary antibody conjugated with a detection marker, and analyze under a microscope. Follow protocol steps for reliable results, accurate interpretation, and overall experiment success. Proper preparation is crucial for accurate results.