Test Preparation : No special preparation required

Sample : Paraffin Blocks/Tissue in buffered formalin

Methodlogy : IHC

Description :

WHAT IS IT?

In pathology and molecular biology, immunohistochemistry (IHC) is a useful method for identifying certain proteins in tissue samples. The IHC marker Ki-67, which assesses cellular proliferation and growth, is often used. During the active stages of the cell cycle, such as G1, S, G2, and mitosis, the Ki-67 protein is present in cells; however, it is lacking in cells that are at rest (G0). Pathologists and researchers can learn more about the growth portion of a tissue sample by using the Ki-67 marker test using IHC, which aids in identifying quiescent cells from actively dividing ones. This knowledge is essential in many clinical and academic contexts, assisting in the evaluation of diseases like cancer, which are characterized by fast cell growth. The Ki-67 marker test aids in assessing the level of cellular proliferation inside a tissue, enhancing diagnostics, prognosis, and patient-specific treatment plans.

WHY?

This test is used to evaluate tumor cell proliferation as well as the prognosis in patients with different forms of cancer. Since Ki-67 is a nuclear non-histone protein, quiescent cells have low amounts of it while proliferating cells have higher levels. Hence, it is referred to be a particular nuclear marker for cell proliferation. A higher Ki-67 reactivity may indicate unfavorable results. If the Ki-67 reading is less than 10%, the prognosis for breast cancer is excellent. Unfavorable when it exceeds 20% and borderline when it is between 10 and 20%.

PRECAUTIONS

For this test, no extra preparation is required. Before having the IHC-Ki-67-Marker, let your doctor know if you use any prescription drugs, have any underlying medical problems, or have any allergies. Your doctor will provide particular advice based on your condition.