Test Preparation : No special preparation required

Sample : Paraffin Blocks/Tissue in buffered formalin

Methodlogy : IHC

Description :

WHAT IS IT?

Pathologists can use the Immunohistochemistry (IHC) Vimentin test as a diagnostic tool to find out whether tissue samples contain the protein vimentin. In mesenchymal cells including fibroblasts, endothelial cells, and certain immune cells, vimentin, an intermediate filament protein, is frequently present. Application of certain antibodies that bind to the vimentin protein in tissue slices is required for the IHC Vimentin test. Pathologists can recognize and locate the vimentin protein inside the tissue by using these antibodies, which are frequently marked with visual markers that can be viewed under a microscope. Vimentin expression is frequently linked to a mesenchymal phenotype, hence the test's results can reveal important details regarding the cellular origin and differentiation state of malignancies. The IHC Vimentin test is essential for the diagnosis and study of cancer because it helps to categorize tumors and advances our understanding of the molecular architecture of tissue.

WHY?

Vimentin immunohistochemistry is a control marker used in the diagnosis of cancer because it may be utilized to identify tumors or carcinomas. A positive identification of the Mesenchymal Origin Tumor is the expected outcome of this test. This is relevant to people of both sexes and of all ages, from infants to seniors. Vimentin staining may be absent to demonstrate that areas that resemble fat or other spaces truly lack a cellular lining. carcinomas are indicated by negative staining. Rheumatoid oncocytoma Thymic cancer, renal chromophobe carcinoma, and thymoma. It's vital to keep in mind that this test occasionally produces both false positives and false negatives.

PRECAUTIONS

Vimentin Immunohistochemistry doesn't necessitate any special setup. However, it's crucial to maintain your composure and let the physicians know about any drugs you've been taking. You will be advised of any changes in your current medication so that the test can produce reliable findings based on them and the time of drug consumption. Prior to the test, let the doctor know if you have any allergies, and make sure to follow all of his or her exact recommendations. No dietary adjustments are necessary.